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. 2022 Apr 26;13:2255. doi: 10.1038/s41467-022-29873-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Whole-cell lipid extracts of M. tuberculosis mc²6230, the Δrv0455c mutant, and the Δrv0455c mutant complemented with an integrated rv0455c expression vector were analyzed by lipidomics using high-performance liquid chromatography and mass spectroscopy. a Chemical structure of the core of M. tuberculosis siderophores. R₁: H or CH₃ represent the serine (S) or methylserine (M) form, respectively. R₂: alkyl chains. The mean intensity values for molecular events are shown for a subset of carboxymycobactin isoforms (b) and mycobactin isoforms (c). The indicated fatty acid chain lengths (number of carbons) and the number of double bonds corresponding to mono- or di-carboxyl units assume the peptide structure shown in (a), which according to prior analyses is the dominant form^12,55. The monodeoxy-mycobactins and monodeoxy-carboxymycobactins are indicated with a hash tag (^#). d Total mycobactin and total carboxymycobactin counts in wt M. tuberculosis (blue), the Δrv0455c mutant ML2203 (red), and the complemented strain ML2205 (gray). Data are mean ± s.d. of four biological replicates. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s test) compared with wt M. tuberculosis. Source data are provided in the Source Data file.