Skip to main content
. 2022 Apr 26;13:2246. doi: 10.1038/s41467-022-29852-x

Fig. 5. The control cohort with α1KD in PV + TRN neurons displayed no changes to NREM or wake time, or delta (δ, 1.5–4 Hz) power in any states, including transitions from NREM to REM, in the light (inactive) period.

Fig. 5

a Examples of frontal electroencephalogram (EEG) and electromyogram (EMG) recordings during a NREM period (ZT0-4) in baseline (BL; blue) and following α1KD (red), from a single mouse. b Compared with their baseline (BL) recordings, α1KD mice had unchanged non-rapid eye movement sleep (NREM delta power ZT0-4, p = 0.2; ZT4-8, p = 0.32; ZT8-12, p = 0.58; ZT12-16, p = 0.42; ZT16-20, p = 0.47; ZT20-24, p = 0.88. Significance was determined using two-tailed paired t-tests. Thick lines indicate mean; envelopes indicate SEM. The shaded area indicates the 12 h dark period. c NREM delta (δ) theta (θ) and sigma (σ) power were not different between BL and α1KD records, shown here is the power profile from NREM in ZT-0-4 when we saw the largest effect in the α3KD mice. Significance was determined using two-tailed paired t-tests. Thick lines indicate mean; envelopes indicate SEM. d Baseline time-frequency power dynamics reveal high delta power in NREM leading to a transition to rapid eye movement (REM) sleep. e After α1KD, the high delta power in NREM before a transition to REM is the same as in baseline records. f Compared with baseline (blue), α1KD (red) mice had unaltered delta power in the NREM before a transition to REM [t (6) = 0.67, p = 0.26]. Significance was tested using a one-tailed paired t-test. Thick lines indicate mean; envelopes indicate SEM. g Baseline time-frequency power dynamics reveals high delta power in NREM leading to a transition to wake. h α1KD did not increase the delta in NREM before a transition to wake. i Compared with baseline (blue), α1KD (red) mice had unchanged delta power in the NREM before a transition to wake [t (6) = −0.36, p = 0.63]. Significance was tested using a one-tailed paired t-test. Thick lines indicate mean; envelopes indicate SEM. n.s. indicates not significant. d–i show data from whole 12 period of light. b–i N = 7. Color scales represent normalized power (power at time/power from wakefulness).