Fig. 4.

CEMIP regulated pro-inflammatory cytokines in human OA FLS RT-qPCR analysis of IL8 (n = 10), CXCL1 (n = 9), IL1B (n = 6) and CEMIP (n = 10) genes in CEMIP-silenced FLS (shCEMIP#1 and shCEMIP#2) compared to non-silenced cells (shEGFP) treated or not with TNF-α for one day (a). ELISA analysis of IL-6 (n = 8) and MMP-3 (n = 6) secretion from CEMIP-silenced FLS (shCEMIP#1 and shCEMIP#2) and non-silenced cells (shEGFP) treated or not with TNF-α for one day (b). Data are expressed as mean with SEM. Parametric paired ANOVA test followed by Tukey post hoc test (for values that pass normality test) or non-parametric paired Friedman test followed by Dunn’s post hoc test (for values that did not pass normality test) were applied. *p < 0.05, **p < 0.01 and ***p < 0.001