Fig. 2. KFE8-Ag85B-pulsed DC delivery increases frequency of total and parenchymal CD4⁺ memory T cells in lungs of BCG-primed mice.

a Surface markers for tissue resident T cells were selected and used to further characterize KFE8-Ag85B-generated T cells as demonstrated in a graphical representation of lung-resident memory T cells produced in BioRender. b Vaccination studies were repeated and the data shown is from lungs of C57BL/6 mice (n = 9–10) harvested 4 weeks post boost. CD3⁺ CD4⁺ CCR7^- cells were gated on CD44^hi and CD62L^lo, which represent total Teff and Tmem cells. Tmem cells were differentiated from Teff cells by the positive expression of CD127, such that Tmem cells are CD44^hi CD62L^lo CD127⁺ and Teff cells are CD44^hi CD62L^lo CD127⁻. Cells were additionally gated on CD45 to determine how cells were distributed between the vasculature (i.v.⁺) and the lung parenchyma (i.v.^-). Tetramer⁺ cells within the Tmem population were also identified. c Frequencies of Teff, Tmem, Tet⁺ Tmem and i.v.^- Tmem were determined from CD44^hi CD62L^lo T cells and expressed as a percentage of the total CD4⁺ T cell population. Data shown as mean ± SE. Significance was determined by one-way ANOVA followed by a Benjamini Krieger Yekutieli post hoc test for multiple comparisons to determine differences due to treatment and among treatment groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.