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. 2022 Apr 13;13:836106. doi: 10.3389/fphys.2022.836106

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Copyright © 2022 Verdonckt and Vanden Broeck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact on total RNA extraction efficiency of three pre-treatments. Concentrated bacterial cells expressing two dsRNA constructs (dsGFP or dsDcr2) were freeze-thawed ten times and then subjected to cell-disruption treatments. The total RNA was then extracted through a phenol/guanidine-based method and the concentration measured through a spectrophotometer. Four replicates were tested for each condition (32 samples in total). Symbols indicate the mean, error bars the standard deviation. All pre-treatments significantly (p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test) increased the extraction yields compared to the control method and differed between each other.