FIGURE 5.

The production yield of dsRNA per ml of bacterial culture is dependent on the pre-treatments applied and the dsRNA construct. The data of Table 2 were sorted as described in Figure 4 to visualize the impact of the experimental variables on dsRNA yield. Symbols indicate the mean, error bars the standard deviation. (A): Purification methods did not impact the final production yield of dsRNA per ml cell culture (p = 0.9935). (B): The pre-treatments had a major effect on the final production yield of dsRNA per ml cell culture (p < 0.0001). (C): The dsRNA construct had an impact on the final production yield of dsRNA per ml cell culture (p = 0.0002). Statistical analysis was performed with a repeated measure two-way ANOVA.