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. 2022 Apr 13;13:836106. doi: 10.3389/fphys.2022.836106

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Copyright © 2022 Verdonckt and Vanden Broeck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacteria-derived dsRNA is effective at inducing a knockdown in the RP-HzGUT-AW1 cell line. Bacteria-derived or MEGAscript produced dsRNA targeting GFP or the Helicoverpa Dicer2 gene was transfected into these gut-derived cells of Helicoverpa zea. Transcript levels were assessed 1 day after treatment. Symbols indicate the mean, error bars the standard deviation. Four replicates were tested per condition. All experimental treatments yielded significant knockdowns as assessed through multiple unpaired parametric t-test (** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001). MEGA = dsRNA produced in vitro by using the MEGAscript kit; B = sonication pre-treatment; C = heating pre-treatment; E = NaCl digested dsRNA; L = LiCl precipitated dsRNA.