TABLE 2.
dsRNA production yield (µg dsGFP or dsDcr2 per ml bacterial culture) for all combinations of pre-treatments and purification methods tested.
| µg/ml dsGFP | Purification method | Average per pre-treatment | ||
|---|---|---|---|---|
| LiCl precipitation | NaCl buffer | TURBO buffer | ||
| Pre-treatment | ||||
| Lysozyme | 3.19 | 3.51 | 3.48 | 3.39 |
| Sonication | 7.98 | 8.06 | 8.09 | 8.04 |
| Heating | 5.56 | 4.82 | 4.38 | 4.92 |
| Control | 2.69 | 2.51 | 2.48 | 2.56 |
| Average per purification method | 4.86 | 4.73 | 4.61 | |
| μg/ml dsDcr2 | ||||
| Pre-treatment | ||||
| Lysozyme | 3.71 | 4.63 | 4.42 | 4.25 |
| Sonication | 9.21 | 9.08 | 9.36 | 9.22 |
| Heating | 6.30 | 5.68 | 6.60 | 6.19 |
| Control | 3.23 | 3.10 | 3.20 | 3.18 |
| Average per purification method | 5.61 | 5.62 | 5.89 | |