Figure 11.

HNF4α expression and function is decreased in OGT-KO mice 28 days after PHX.A, Western blot analysis of HNF4α protein levels in livers of control and OGT-KO mice 28 days after PHX, with corresponding densitometry. B, Representative photomicrographs (400×) of HNF4α IHC of control and OGT-KO mice 28 days post PHX. qPCR analysis of HNF4α-positive target genes (C) and HNF4α-negative target genes (D) at 28 days after PHX. qPCR was normalized to 18S and median of the control group. Bars represents the mean ± standard deviation. ∗P < .05; ∗∗P < .01; and ∗∗∗∗P < .0001. E, IP pull-down of HNF4α from WT and OGT-KO mice. Western blot analysis was performed on HNF4α to show successful pulldown (upper blot) and O-GlcNAcylation (lower blot).