Supplementary Table 5.
List of Primer Sets Used for ChIP DNA qPCR
| Species | Gene locus | Forward primer | Reverse primer | Probe |
|---|---|---|---|---|
| Mouse | Pdx1 A-I | CCAGTATCAGGGAGGACTATCA | TACCCAGCCATTAGGCAAGA | |
| Mouse | Pdx1 A-III | ACCGTGTCACCAAGTCAACCC | AGAGCCACCTGTGCCCGTCAA | |
| Mouse | Pdx1 A-IV | CTCTTCCTGATTCCCTGAAGTC | ACTAAGAGTGCTCTGGGCTCTG | |
| Human | INS pro | GTGGAAAGTGGTTTAGGTGAGGGT | ACCTGCTTGATGGCCTCTTCTGAT | |
| Human | PDX1 pro | CACACAACGAATGCCAGAGTTTCG | ACTGATCTCAGAGGGAACCCACA | |
| Human | NEUROG3 pro | AAGAGAGGCAGTGAAACACCAGGA | AACTCTCGGTTCCTCAAAGAGCCT | |
| Human | HES1 pro | TCCTCCTCCCATTGGCTGAAAGT | TTGGTGATCAGTAGCGCTGTTCCA | |
| Human | INS -275 | TGTGAGCAGGGACAGGTCTG | TCCTCAGGACCAGCGGG | 6FAM-CCACCGGGCCCCTGGTTAAGACTCTA |
| Human | INS +1318 | CAGCTGGAGAACTACTGCAACTAGA | GCTGGTTCAAGGGCTTTATTCC | 6FAM-CCGCCTCCTGCACCGAGAGAGA |
NOTE. Sequences start from 5' to 3'. Gene loci for which a probe sequence is not provided were performed using SYBR green chemistry. TaqMan qPCR was used for human INS-275 and INS+1318.
Pro, promoter.