Fig. 2. The chromatographic fractionation curve of labeled ¹²⁵I-OPN obtained via the iodogen method (a), the initial adsorption and final retention amounts of ¹²⁵I-OPN on various SAMs (b), the binding domains of OPN on each SAM evaluated via ELISA (c), and the unit mass values of bound mAbs on the indicated surfaces (d). In (b), initial adsorption was calculated after rinsing with PBS, while final retention was obtained after further treatment with complete culture medium. The stars indicate significant difference (p < 0.05).