Experimental range and levels coded for ANOVA.
| Factor | Unit | Range and levels | ||||
|---|---|---|---|---|---|---|
| −2 | −1 | 0 | +1 | +2 | ||
| IPTG concentration | mg L−1 | 0 | 20 | 40 | 60 | 80 |
| Induced cell density | — | −0.3 | 0.2 | 0.7 | 1.2 | 1.7 |
| Induction temperature | °C | 0 | 10 | 20 | 30 | 40 |
| Induction culture time | h | 0 | 4 | 8 | 12 | 16 |
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