| Culture supernatant containing Rpf (SRpf) from M. luteus
|
The strain of M. luteus was incubated in a lactate minimal medium (LMM) on a rotary shaker (160 rpm, 30 °C) for 36 h, and then was inoculated to fresh LMM and incubated under the same culture condition until a stationary phase was achieved. Finally, the culture supernatant was obtained by centrifugation and filtration |
Biphenyl degradation |
Biphenyl at concentration of 1500 mg L−1 was almost completely degraded in 24 h using SRpf at a dosage of 15% (v/v) |
51 and 54
|
| Recombinant protein RpfSm (a truncated form of Rpf from M. luteus) |
The strain of E. coli was first grown to an OD600nm = 0.65–0.8 and induced with 1 mM IPTG for 2 h at room temperature |
Resuscitate and stimulate gram-positive bacteria |
Addition of the recombinant Rpf protein (15 μg mL−1) resulted dispersion of cell aggregates and emergence of solitary cells |
55
|
| Purified recombinant Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were cultured with 50 μg mL−1 kanamycin and induced by 100 μg mL−1 IPTG. Then, the culture supernatant obtained by centrifugation and sonication was applied to the Ni-NTA-agarose column. Finally, the eluate was dialyzed against 25 mM Tris–HCl at 4 °C for 17–24 h |
Salt-tolerant phenol degradation |
Rpf at a dosage of 1% (v/v) accelerated the start-up process during sludge domestication with higher concentrations of phenol (1500 mg L−1) and NaCl (30 g L−1) |
17
|
| Purified recombinant Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were cultured with 50 μg mL−1 kanamycin and induced by 100 μg mL−1 IPTG. Then, the culture supernatant obtained by centrifugation and sonicatation, and was applied to the Ni-NTA-agarose column. Finally, the eluate was dialyzed against 25 mM Tris–HCl at 4 °C for 17–24 h with the concentration of 0.7218 g L−1
|
Cellulose-degrading of the bacterial community in composting |
The activity of filter paper cellulose and carboxymethyl cellulase increased 0.1028 IU mL−1 and 0.0.1282 IU mL−1 in the treatment group with 0.25% Rpf addition (v/v) |
21
|
| Purified recombinant Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were cultured with 50 μg mL−1 kanamycin and induced by 100 μg mL−1 IPTG. Then, the culture supernatant obtained by centrifugation and sonication, and was applied to the Ni-NTA-agarose column. Finally, the eluate was dialyzed against 25 mM Tris–HCl at 4 °C for 17–24 h |
Treatment of high-saline phenolic wastewater in MBR system |
Phenol removal of sludge with Rpf addition (1%, v/v) was more than twice as that without Rpf in the MBR system |
18
|
| Culture supernatant containing Rpf (SRpf) from M. luteus
|
The strain of M. luteus was incubated in lactate minimal medium (LMM) on a rotary shaker (160 rpm, 30 °C) for 36 h, and then was inoculated to fresh LMM and incubated under the same culture condition until stationary phase. Finally, the culture supernatant was obtained by centrifugation and filtration |
Biological nutrient removal in SBR process |
PO43−-P removal efficiency increased by over 12% and total nitrogen removal efficiency increased by over 8% in the treatment reactor acclimated with SRpf addition (10%, v/v) |
20 and 54
|
| Purified recombinant Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were cultured with 50 μg mL−1 kanamycin and induced by 100 μg mL−1 IPTG. Then, the culture supernatant obtained by centrifugation and sonication, and was applied to the Ni-NTA-agarose column. Finally, the eluate was dialyzed against 25 mM Tris–HCl at 4 °C for 17–24 h |
Nitrogen removal |
Strain SSPR1 resuscitated by Rpf (3%, v/v) showed high NH4+ removal efficiency and the removal efficiency reached 72.3% after 72 h |
23
|
| Extracellular organic matter (EOM) from M. luteus
|
The strain of M. luteus was incubated in lactate minimal medium (LMM) on a rotary shaker (160 rpm, 30 °C) for 36 h, and then was inoculated to fresh LMM and incubated under the same culture condition until stationary phase was obtained. Finally, the culture supernatant was obtained by centrifugation and filtration |
Biphenyl degradation |
Under a concentration of 3,500 mg L−1 biphenyl, biphenyl degradation efficiency reached 60.8% at a dosage of 10% EOM (v/v) |
50 and 54
|
| Crude Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were grown in a 2×YT medium broth with 50 μg mL−1 kanamycin and 100 μg mL−1 IPTG. After centrifugation and sonication, the supernatant was then filtered through a 0.22 mm filter |
Biodegradation of polychlorinated biphenyls (PCBs) |
In soil microcosms containing 50 mg kg−1 Aroclor 1242 and inoculated with VBNC TG9T cells, after 49 d of supplementation with Rpf 20% (v/v), degradation efficiency of PCB reached 34.2% |
56
|
| Crude Rpf protein from M. luteus
|
The E. coli BL21 (DE3) cells were cultured with 50 μg mL−1 kanamycin and induced by 100 μg mL−1 IPTG. Then, the culture supernatant obtained by centrifugation and sonication, and was applied to the Ni-NTA-agarose column. Finally, the eluate was dialyzed against 25 mM Tris–HCl at 4 °C for 17–24 h |
Degradation of reactive blue 19 |
The strain Bacillus sp. JF4 resuscitated by Rpf addition (0–1.6%, v/v) could effectively decolorize RB19 |
57
|
