figs1.

Supplementary Fig. 1; The knockdown efficiency of siHIF-1⍺ and the expression of PMAN in gastric cancer peritoneal metastases.a,b The expression level of HIF-1α mRNA was detected by RT-qPCR (a) and protein was analyzed by western blotting (b) in AGS cells transfected with siHIF-1⍺#1, siHIF-1⍺#2 or siNC during hypoxia. c Ranking of the top ten lncRNA genes associated with HIF-1α, expression as assessed by lncRNA microarray analysis and lncRNA-seq results. d RT-qPCR detects the expression of PMAN in AGS cells transfected with siHIF-1⍺#1, siHIF-1⍺#2 or siNC during hypoxia. According to the results,siHIF-1⍺#1 was selected for follow-up experiments. e Kaplan-Meier curves for overall survival rates correlated with HIF-1α expression in STAD from GEPIA database. f RT-qPCR detects the expression of PMAN in GC cells. According to the expression level of PMAN and cell state, AGS and MGC-803 cells were selected for follow-up experiments. g The expression of PMAN after transfected with shPMAN#1, shPMAN#2, shPMAN#3 or shNC were detected by RT-qPCR. According to the results shPMAN#2 was selected for follow-up experiments. h Fish showed the expression of PMAN (red) in 3 pairs GC peritoneal metastasis samples. The nucleus was stained with DAPI. Scale bar, 20 μm i Immunohistochemistry was used to analyze up-regulated HIF-1α expressions in paired GC peritoneal metastasis samples. Scale bar, 20 μm j ISH showed the expression of PMAN in 3 pairs GC peritoneal metastasis samples. Scale bar, 20 μm. Mean ± SD is shown. Statistical analysis was conducted using one-way ANOVA. Ns = nonsignificant (p > 0.05), *p < 0.05, **p < 0.01.