TABLE 3.

Location and evidence for MDR deletions in E. coli^a

Operon	E. coli contig accession no.	Location of operon in contig (nt)b	Location of deletion in contig (nt)	Predicted amplification product (kbp)c
				WTd	Deletion strain
tolC	AE000385	5769–7256	5810–7206	5.7	5.8
yjcP	AE000481	9588–8122	8153–9502	5.8	5.9
yohG	AE000303	3588–2392	2512–3490	5.4	5.9
ylcB	AE000162	2377–3750	2402–3648	5.6	5.8
acrAB	AE000152	9033–4668	4721–8937	8.5	5.7
acrEF	AE000405	3660–7933	3585–7872	9.5	6.5
yhiUV	AE000427	8349–12644	8432–12593	8.5	5.7
acrD	AE000334	112–3225	139–3163	7.6	6.0
yegMNO	AE000297	7262–14856	7317–14737	11.5	5.6
emrD	AE000445	1091–2281	1114–2029	5.2	5.7
mdfA	AE000186	5562–6794	5637–6720	5.2	5.5
emrAB	AE000353	1902–4629	2018–4562	6.5	5.5
emrKY	AE000325	2785–84	149–2716	6.7	5.5
emrE	AE000160	1663–1995	1718–1974	4.5	5.7
tehAB	AE000240	1932–3514	1990–3411	5.5	5.5
ybjYZ	AE000189	8123–11208	8157–11189	7.1	5.5

^aEvidence for replacement of operon with the Km^r marker, based on predicted amplification product sites. 

^bnt, nucleotides. 

^cAmplification products were predicted based on location of primers located outside the DNA necessary for gene replacement (see Materials and Methods). 

^dWT, wild type.