TABLE 4.
Susceptibility of E. coli MDR gene deletion strains to toxic compounds
| W3110 pump deletion strain | Strain no. | MDR family | MIC (μg/ml)
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMP | CAM | FLOR | CLO | PUR | ERM | METH | NOV | CPRO | NOR | NAL | RIF | FUS | SM | SUL | |||
| W3110 | HS414 | 12.5 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 3.125 | 5 | 400 | 1.95 | 2,000 | |
| ΔtolC::Kmr | HS151 | OMF | 6.25 | 0.78 | 0.78 | 1 | 1.56 | 1.56 | 20 | 0.78 | 0.0025 | 0.001 | 0.39 | 10 | 3.125 | 1.95 | 2,000 |
| ΔyjcP::Kmr | HS154 | OMF | 12.5 | 6.25 | 6.25 | >32 | 25 | 25 | >640 | 100 | 0.01 | 0.004 | 3.125 | 5 | 400 | 1.95 | 2,000 |
| ΔyohG::Kmr | HS157 | OMF | 12.5 | 6.25 | 3.12 | >32 | 25 | 50 | >640 | 100 | 0.01 | 0.004 | 3.125 | 5 | 400 | 1.95 | 2,000 |
| ΔylcB::Kmr | HS208 | OMF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.002 | 6.25 | 2.5 | 400 | 1.95 | 2,000 |
| ΔyjcP ΔyohG ΔylcB ΔtolC::Kmr | HS230 | OMF | 3.12 | 0.78 | 0.78 | 1 | 1.56 | 1.56 | 20 | 0.78 | 0.0025 | 0.001 | 0.39 | 10 | 3.125 | 1.95 | 2,000 |
| ΔacrAB::Kmr | HS832 | RND | 3.12 | 0.78 | 0.78 | 16 | 1.56 | 1.56 | 80 | 1.56 | 0.0025 | 0.004 | 1.56 | 2.5 | 3.125 | 1.95 | 2,000 |
| ΔacrEF::Kmr | HS212 | RND | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔyhiUV::Kmr | HS193 | RND | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔacrD::Kmr | HS215 | RND | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔyegMNO::Kmr | HS199 | RND | 12.5 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.02 | 0.004 | 6.25 | 5 | 800 | 3.9 | 2,000 |
| ΔacrEF ΔacrAB ΔyhiUV ΔacrD ΔyegMNO::Kmr | HS276 | RND | 1.56 | 0.78 | 0.78 | 2 | 1.56 | 1.56 | 20 | 0.39 | 0.0025 | 0.001 | 1.56 | 2.5 | 3.125 | 1.95 | 2,000 |
| ΔemrD::Kmr | HS196 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 3.125 | 2.5 | 400 | 1.95 | 2,000 |
| ΔmdfA::Kmr | HS221 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 2.5 | 400 | 1.95 | 2,000 |
| ΔemrD ΔmdfA::Kmr | HS235 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 3.125 | 2.5 | 400 | 1.95 | 2,000 |
| ΔemrAB::Kmr | HS833 | MF | 12.5 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔemrKY::Kmr | HS205 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔemrKY | HS236 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔemrKY ΔemrAB ΔemrD ΔmdfA::Kmr | HS275 | MF | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔemrE::Kmr | HS218 | SMR | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 2.5 | 400 | 1.95 | 2,000 |
| ΔtehAB::Kmr | HS202 | SMR | 12.5 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 400 | 0.01 | 0.004 | 6.25 | 10 | 400 | 1.95 | 2,000 |
| ΔemrE ΔtehAB::Kmr | HS238 | SMR | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 5 | 400 | 1.95 | 2,000 |
| ΔybjYZ::Kmr | HS273 | ABC | 6.25 | 6.25 | 6.25 | >32 | 100 | 50 | >640 | 100 | 0.01 | 0.004 | 6.25 | 2.5 | 400 | 1.95 | 2,000 |
| MIC (μg/ml)
| |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TET | DOC | CHO | TDOC | SDS | OXAL | PROF | CV | ACR | EBR | TPP | R6G | TPAC | CTAB | DEQC | BENZ | DAUN | PLUM | VIOL | CCCP |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 800 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 40 | 50 | 26 |
| 0.156 | 200 | 1,600 | 400 | 50 | >3,200 | 6.25 | 0.78 | 3.12 | 3.12 | 6.25 | 2.5 | 1.56 | 25 | 25 | 2 | 0.625 | 10 | 50 | 1.6 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 50 | 25 | 100 | 400 | 800 | 1,280 | 400 | 100 | 160 | 31.2 | >80 | 40 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 50 | 25 | 100 | 400 | 800 | 1,280 | 400 | 100 | 160 | 31.2 | >80 | 40 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 26 |
| 0.156 | 200 | 1,600 | 400 | 50 | >3,200 | 12.5 | 0.78 | 3.12 | 3.12 | 6.25 | 2.5 | 3.125 | 25 | 2.5 | 2 | 0.625 | 10 | 50 | 1.6 |
| 0.156 | 1,600 | >12,800 | >12,800 | 100 | >3,200 | 12.5 | 0.78 | 3.12 | 3.12 | 6.25 | 2.5 | 3.125 | 2.5 | 2.5 | 2 | 0.625 | 10 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 800 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 52 |
| 0.156 | 1,600 | 6,400 | 400 | 100 | >3,200 | 6.25 | 0.78 | 3.12 | 1.56 | 6.25 | 2.5 | 1.56 | 25 | 2.5 | 2 | 0.625 | 10 | 25 | 26 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 200 | 1,600 | 1,280 | 1,600 | 100 | 160 | 15.6 | >80 | >80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 200 | 1,600 | 1,280 | 1,600 | 100 | 160 | 15.6 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 800 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 40 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 200 | 200 | 1,600 | 1,280 | 1,600 | 100 | 160 | 15.6 | >80 | 80 | 50 | 13 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 50 | 200 | 1,600 | 1,280 | 1,600 | 100 | 160 | 62.5 | >80 | 80 | 6.25 | 13 |
| 1.25 | >3,200 | 12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 800 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 100 | 26 |
| 1.25 | >3,200 | >12,800 | >12,800 | >12,800 | >3,200 | 100 | 25 | 50 | 200 | 1,600 | 1,280 | 1,600 | 100 | 160 | 62.5 | >80 | 80 | 6.25 | 13 |
| 1.25 | >3,200 | >12,800 | 12,800 | >12,800 | >3,200 | 100 | 25 | 400 | 400 | 1,600 | 1,280 | 1,600 | 200 | 160 | 62.5 | >80 | 80 | 50 | 13 |
Values in bold differ from the W3110 control strain value by ≥4-fold.
Abbreviations: AMP, ampicillin; CAM, chloramphenicol; FLOR, florfenicol; CLO, clotrimazole; PUR, puromycin; ERM, erythromycin; METH, methotrexate; NOV, novobiocin; CIPRO, ciprofloxacin; NOR, norfloxacin; NAL, nalidixic acid; RIF, rifampin; FUS, fusidic acid; SM, streptomycin; SUL, sulfacetamide; TET, tetracycline; DOC, deoxycholate; CHO, sodium cholate; TDOC, sodium taurodeoxycholate; OXAL, sodium oxalate; PROF, proflavin; CV, crystal violet; ACR, acriflavin; EBR, ethidium bromide; TPP, tetraphenylphosphonium; R6G, rhodamine 6G; TPAC, tetraphenylarsonium chloride; DEQC, dequalinium chloride; BENZ, benzalkonium chloride; DAUN, daunomycin; PLUM, plumbagin; VIOL, methyl viologen.