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. 2022 Feb 15;13(5):1554–1589. doi: 10.1016/j.jcmgh.2022.02.007

Figure 1.

Figure 1

Sample processing. (A) Schematic for isolating single epithelial cells from 6 intestinal regions for 3 donors and then using hashtag antibodies to sequence cells from all regions side-by-side. (B) UMAP of all analyzed cells in 25 lineage clusters. See Figure 19 for clearly marked clusters. (C–E) UMAP of all cells colored by (C) donor or (D and E) region. (F and G) Heatmaps showing unique markers for major lineages in (F) SI and (G) colon. See Supplementary Table 1 for total DEGs for each lineage. Schematics in panel A were created with BioRender.com. Duo, Duodenum; FACS, fluorescence-activated cell sorter; Ile, Ileum; Interm, intermediate; Jej,Jejunum; Sec. Prog., Secretory Progenitor; UMAP, Uniform Manifold Approximation and Projection.