Figure 20.

Hashtag deconvolution. (A) Per donor hashtag noise distributions. Blue dotted lines indicate the 99th percentile values for noise. Values above this line were called positive for a specific hashtag. (B) Left: K-medoid clustering for each donor based only on hashtag reads. Cells positive (P < .01) for multiple hashtags were removed as likely multiplets. Cells were called as negative if they did not surpass the noise threshold for all hashtags. Right: K-medoid clustering with final hashtag labeling for nonmultiplet cells.