Table 1.
Reagents Used
| Company | Catalog number | |
|---|---|---|
| Reagent | ||
| N-acetylcholine | Millipore Sigma (St. Louis, MO) | A9165 |
| dPBS | Gibco (Jenks, OK) | 14190-144 |
| Na2HPO4 | Millipore-Sigma | S7907 |
| KH2PO4 | Millipore Sigma | P5655 |
| NaCl | Millipore Sigma | S5886 |
| KCl | Millipore Sigma | P5405 |
| Sucrose | Fisher Scientific (Hampton, NH) | 220-1 |
| D-sorbitol | Fisher Scientific | 439-500 |
| Y27632 | Selleck Chemical (Houston TX) | S6390 |
| EDTA | Corning (Corning, NY) | 46-034-Cl |
| Dithiothreitol | Fisher Scientific | 172-5 |
| Protease VIII | Millipore Sigma | P5380 |
| Advanced DMEM/F12 | Gibco | 12634-010 |
| Bovine serum albumin | Fisher Scientific | BP1600-1 |
| TotalSeq anti-human hashtag antibodies | BioLegend (San Diego, CA) | B0251-B0256 |
| 10% neutral buffered formalin | Fisher Scientific | 22-050-105 |
| Histo-clear | National Diagnostics (Atlanta, GA) | HS2001 |
| Triton X-100 | MP Biomedicals (Irvine, CA) | 02194854-CF |
| Prolong Gold Antifade Reagent | Invitrogen (Waltham, MA) | P36930 |
| Xylenes | Millipore-Sigma | 534056 |
| RNAscope Multiplex Fluorescent Reagent Kit v2 | Advanced Cell Diagnotics (Newark, CA) | 323100 |
| Antibodies | ||
| AnnexinV-APC | BioLegend | 640920 |
| Lysozyme | Diagnostic Biosystems (Pleasanton, CA) | RP028 |
| CFTR | Cystic Fibrosis Foundation | A570 |
| Mucin 2 (Ccp58) | Santa Cruz Biotechnology (Dallas, TX) | sc-7314 |
| RAB27A | Proteintech (Rosemont, IL) | 17817-1-AP |
| MUC5B | Millipore Sigma | HPA008246 |
| FKBP1A | Thermo Fisher (Waltham, MA) | PA1-026A |
| IMPDH2 | Proteintech | 12948-1-AP |
| DHFR | Proteintech | 15194-1-AP |
| Ki-67 monoclonal antibody (SolA15), APC | Invitrogen | 17-5698-82 |
| Donkey anti-rabbit IgG (H+L), Alexa Fluor 488 | Invitrogen | A-21206 |
| Cy3 AffiniPure F(ab')2 fragment donkey anti-rabbit IgG (H+L) | Jackson Immunoresearch (West Grove, PA) | 711-166-152 |
| Alexa Fluor 647 AffiniPure donkey anti-mouse IgG (H+L) | Jackson Immunoresearch | 715-605-150 |
| Bisbenzamide | Millipore Sigma | 14530 |
| RNAscope probes | ||
| Hs-SMOC2 | Advanced Cell Diagnotics | 533921 |
| Hs-BEST4 | Advanced Cell Diagnotics | 481501 |
| TSA cyanine 3 reagent pack | Akoya Biosciences (Marlborough, MA) | SAT704A001EA |
| TSA cyanine 5 reagent pack | Akoya Biosciences | SAT715A001EA |
DMEM, Dulbecco’s modified Eagle medium.