Figure 2.

Effect of ligustilide on HIF-1α and c-Myc protein levels in hypoxic TW2.6 cells. (A) TW2.6 cells were treated with ligustilide and incubated at 37°C of hypoxia for 24 h. Cells were harvested and protein was analyzed by Western blot. A normoxia (N) group was performed as a control group compared to hypoxia. (B) Cells were treated with ligustilide in different doses and incubated at 37°C of hypoxia for 24 h. Protein (left) and mRNA (right) were harvested and analyzed. Data in the plot are expressed as mean ± SEM (n ≥ 3). *P-valve < 0.05; ***P-valve < 0.001.