Figure 3.

Effects of ligustilide on c-Myc mRNA and ubiquitinated c-Myc levels in hypoxic TW2.6 cells. (A) TW2.6 cells were treated with ligustilide and incubated for 24 h of hypoxia at 37°C. Messenger RNA and protein were harvested and c-Myc mRNA was measured (left). Ubiquitinated c-Myc levels were measured by immunoprecipitating c-Myc with anti-c-Myc antibody followed by detection with specific anti-ubiquitin or anti-c-Myc antibodies (right). Data in the plot are expressed as mean ± SEM (n ≥ 3). (B) Effects of ligustilide on ER stress-related signaling in hypoxic TW2.6 cells. Cells were treated with ligustilide in different doses and incubated at 37°C under hypoxia for 24 h. Protein was harvested and analyzed by Western blot.