REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD3 (17A2)	BioLegend	Cat#100204; RRID: AB_312661 Cat#100236; RRID: AB_2561456
Anti-mouse CD4 (RM4-5)	BioLegend	Cat#100406; RRID: AB_312691 Cat#100412; RRID: AB_312697
Anti-mouse CD8a (53-6.7)	BioLegend	Cat#100706; RRID: AB_312745
Anti-mouse TCRαβ (H57-597)	BioLegend	Cat# 109206; RRID: AB_313429
Anti-mouse TCRγδ, (GL3)	BioLegend	Cat# 331208; RRID: AB_1575108
Anti-mouse CD11b (M1/70)	BioLegend	Cat# 151503; RRID: AB_2617034
Anti-mouse CD11c (N418)	BioLegend	Cat# 117306; RRID: AB_313775
Anti-mouse F4/80 (BM8)	BioLegend	Cat# 123108; RRID: AB_893502
Anti-mouse Ly6G (1A8)	BioLegend	Cat# 116206; RRID: AB_313775
Anti-mouse TER119 (TER-119)	Biolegend	Cat# 116206; RRID: AB_313707
Anti-mouse FcεRIa (MAR-1)	Thermofisher	Cat# 11-5898-82; RRID: AB_465308
Anti-mouse CD19 (1D3/CD19)	BioLegend	Cat# 152404; RRID: AB_2629813
Anti-mouse B220(RA3-6B2)	BioLegend	Cat# 103206; RRID: AB_312991
Anti-mouse CD49b (DX5)	BioLegend	Cat# 103504; RRID: AB_313027
Anti-mouse CD45.2 (104)	BD Biosciences BioLegend	Cat# 560693; RRID: AB_1727491 Cat# 109831, RRID: AB_10900256)
Anti-mouse CD45.1	BD Biosciences	Cat# 561235; RRID: AB_10611577
Anti-mouse CD90.2 (30-H12)	BioLegend	Cat# 105320; RRID: AB_493725 Cat# 105328,;RRID: AB_10613293
Anti-mouse CD127 (A7R34)	Thermofisher	Cat# 47-1271-82; RRID: AB_1724012
Anti-mouse NK1.1 (PK136)	BioLegend BD Biosciences	Cat# 108706; RRID: AB_313393 Cat# 564143; RRID: AB_2738617
Anti-mouse IL-18Rα (P3TUNYA),	Thermofisher	Cat# 48-5183-82; RRID: AB_1724012
Anti-mouse ST2 (RMST2-2)	BioLegend Thermofisher	Cat# 145313; RRID: AB_2687364 Cat# 145305; RRID: AB_2561916 Cat# 63-9335-80 RRID: AB_2717062)
Anti-mouse CD226 (10E5)	BioLegend	Cat# 133611; RRID: AB_2715975
Anti-mouse CD49a (Ha31/8)	BD Biosciences	Cat# 564863; RRID: AB_2738987
Anti-mouse NKp46 (29A1.4)	BD Biosciences	Cat# 565099; RRID: AB_2739067
Anti-mouse GATA3 (L50-823)	Thermofisher BD Biosciences	Cat# 56-9966-41; RRID: AB_2811898 Cat# 48-9966-41; RRID: AB_2811833 Cat# 560068; RRID: AB_1645316
Anti-mouse T-bet (4B10)	Thermofisher	Cat# 45-5825-80; RRID: AB_953658 Cat# 12-5825-82; RRID: AB_925761 Cat# 25-5825-82; RRID: AB_11042699
Anti-mouse RORγt (Q31-378)	BD Biosciences	Cat# 562684; RRID: AB_2651150
Anti-mouse TCF-1 (S33-966)	BD Biosciences	Cat# 566693; RRID: AB_2869823
Anti-mouse Arg1 (A1exF5)	Thermofisher	Cat# 56-3697-82; RRID: AB_2734833
Anti-mouse Ki-67 (SolA15)	Thermofisher	Cat# 56-5698-80; RRID: AB_2637479
Anti-mouse Eomes (Dan11mag)	Thermofisher	Cat# 50-4875-82; RRID: AB_2574227
Anti-mouse NK1.1 (PK136)	Bio X Cell	Cat# BE0036; RRID: AB_1107737
Anti-mouse IFN-g	Bio X Cell	Cat# BE0055; RRID: AB_1107694
Anti-mouse CD16/32	BioLegend	Cat# 101320; RRID: AB_1574975
Anti-mouse CD45.2	BioLegend	Cat# 109831; RRID: AB_10900256 Cat# 105320; RRID: AB_493725
Anti-puromycin antibodies (Clone R4743L-E8)	CIML, Marseille	RRID: AB_2827926
Experimental models: Organisms/strains
Mouse C56BL/6	Charles River France	C57BL/6N
Mouse Rag2−/− (B6.129-Rag2Tm1Fwa)	Jackson Laboratories	Cat# 008449 RRID:IMSR_JAX:008449
Mouse Rag2−/−IL2Rg−/- (C; 129S4-Rag2tm1.1Flv Il2rgtm1.1Flv/J) on	Jackson Laboratories	Cat#:014593 RRID:IMSR_JAX:014593
Mouse STAT1−/− (B6.129S(Cg)-Stat1tm1dlv/J)	Jackson Laboratories	Cat#:012606 RRID:IMSR_JAX:012606
Mouse IL12p40−/− (B6.129S1-Il12btm1Jm/J)	Jackson Laboratories	#:002693 RRID:IMSR_JAX:002693
Mouse Red5 (B6(C)-Il5tm1.1(icre)Lky/J)n	Jackson Laboratories	Cat.#:030926 RRID:IMSR_JAX:030926
Mouse ROSA26-YFP (B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J)	Jackson Laboratories	Cat#:006148 RRID:IMSR_JAX:006148
Mouse Id2creERT2 (B6.129S(Cg)-Id2tm1.1(cre/ERT2)Blh/ZhuJ)	Jackson Laboratories	Cat#:016222 RRID:IMSR_JAX:016222
Mouse Rosa26-RFP (Gt(ROSA)26Sortm1Hjf)		MGI:3696099
Mouse mTORfl/fl (B6.129S4-Mtortm1.2Koz/J)	Jackson Laboratories	Cat#:011009 RRID:IMSR_JAX:011009
Mycobacterium tuberculosis/H37Rv	ATCC	Cat# 27294
BCG Danish	ATCC	Cat# 35733
Software and algorithms
FlowJo Software	Treestar	RRID:SCR_008520
BD FACSDiva Software	BD	RRID:SCR_001456
GraphPad Prism V9.2	GraphPad	https://www.graphpad.com
Chemicals, peptides, and recombinant proteins
2 deoxy-glucose (2-DG)	Sigma	Cat# D8375
rmIL-33 (recombinant mouse interleukin-33)	Ozyme	Cat# BLE580504
rmIFNg (recombinant mouse interferon-gamma)	Ozyme	Cat# BLE575306
rmIL-12 (recombinant mouse interleukin-12)	R&D Systems	Cat# 419-ML-050
rmIL-18 (recombinant mouse interleukin-18)	R&D Systems	Cat# 9139-IL-050
rmIL-7 (recombinant mouse interleukin-7)	R&D Systems	Cat# 407-ML-025
rmIL-2 (recombinant mouse interleukin-2)	R&D Systems	Cat# 402-ML-100/CF
Neuromedin U (NMU)	US Biological	Cat# N2171-80E
Live/Dead Fixable Blue	Invitrogen	Cat# L23105
RPMI	Fisher Scientific	Cat# 11544526
Middlebrook 7H9 medium	Difco/Fisher Scientific	Cat# 11753473
Middlebrook 7H11 agar medium	Difco/Fisher Scientific	Cat# 11799042
Albumin-dextrose-catalase, ADC	Difco/Fisher Scientific	Cat# 11718173
Glucose	Sigma-Aldrich	Cat# G7021-5KG
Tamoxifen	Sigma-Aldrich	Cat# T5648
Paraformaldehyde	Fisher Scientific	Cat# 11586711
Collagenase D	Sigma-Aldrich	Cat# 11088882001
DNAse	Sigma-Aldrich	Cat# 10104159001
PMA	Sigma-Aldrich	Cat# P8139-5MG
Ionomycin	Sigma-Aldrich	Cat# I0634-1MG
Critical commercial assays
EasySepTM Mouse ILC2 Enrichment Kit	Stem Cell Technologies	Cat# 19842
Foxp3 transcription factor staining buffer set	ThermoFisher	Cat# 00-5523-00
IFNg ELISA kit	ThermoFisher	Cat# 15511107