REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse monoclonal anti-Fasciclin 3 (7G10) (1:50)	Developmental studies hybridoma bank (DSHB)	RRID: AB_528238
mouse monoclonal anti-Eyes absent (10H6) (1:10)	DSHB	RRID: AB_528232
rat anti-shg/DE-Cadherin (DCAD2) (1:20)	DSHB	RRID: AB_528120
mouse anti-Hts (1B1) (1:10)	DSHB	RRID: AB_528070
mouse anti-Ptc (Apa 1.3) (1:500)	DSHB	RRID: AB_528441
mouse anti-LamDm0 (ADL101) (1:50)	DSHB	RRID: AB_528332
mouse anti-LamC (LC28.26) (1:50)	DSHB	RRID: AB_528339
rabbit anti-Vasa (d260) (1:100)	Santa Cruz Biotechnology	Cat#sc-30210; RRID: AB_793874
rabbit anti-pSmad1/5 (41D10) (1:100)	Cell Signaling Technology	Cat#9516T; RRID: AB_491015
chicken anti-Green Fluorescent Protein (GFP-1020) (1:1000)	Aves Labs	Cat#GFP-1020; RRID: AB_10000240
rabbit anti-P-4E-BP1 (Thr37/46 236B4, 2855S) (1:500)	Cell Signaling Technology	Cat#2855; RRID: AB_560835
rabbit anti-Zfh1 (1:500)	Ruth Lehmann (New York University)	N/A
rabbit anti-Stat92E (1:800)	Denise Montell (UCSB)	N/A
guinea pig anti-Traffic Jam (1:100)	Dorothea Godt (U. Toronto, Canada)	RRID: AB_2568583
Mouse monoclonal anti-phospho-Histone H3 (Ser10) (6G3)	Cell Signaling Technology	Cat#9706; RRID: 331748
Chemicals, peptides, and recombinant proteins
Rapamycin	TSZCHEM	Cat#R1017; CAS#53123-88-9
Trizol	Life Technologies	Cat#15596026
Linear Poly-Acrylamide	Sigma	Cat#56575
DNase Q1	Promega	Cat# M610A
iScriptkit	Biorad	Cat#170-8841
Sso Advanced SYBR Green	Biorad	Cat#1725-264
Critical commercial assays
Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 555 dye	Invitrogen/ThermoFisher	Cat# C10638
Deposited data
Dam:Esg raw array data	This paper	GEO: GSE157685
Experimental models: Cell lines
D. melanogaster: Cell line S2: S2- NLAP	(Voog et al., 2014)	N/A
D. melanogaster: Cell line S2: S2- NLAP:Esg	(Voog et al., 2014)	N/A
D. melanogaster: Cell line S2: S2- NLAP:EsgG387E	(Voog et al., 2014)	N/A
Experimental models: Organisms/strains
D. melanogaster: tub-Gal80TS	Bloomington Drosophila Stock Center	BDSC:7108
D. melanogaster: UAS-InRRNAi [JF01482]	Bloomington Drosophila Stock Center	BDSC:31037
UAS-esgRNAi[HMS02538]	Bloomington Drosophila Stock Center	BDSC:42846
D. melanogaster: D. melanogaster: UAS-InRA1325D (“InRCA”)	Bloomington Drosophila Stock Center	BDSC:8440
D. melanogaster: UAS-IVS-myr::TdTomato	Bloomington Drosophila Stock Center	BDSC:32221
D. melanogaster: UAS-ImpL2RNAi[GD6004]	Vienna Drosophila RNAi Center	v30930
D. melanogaster: UAS-esgRNAi[GD1437]	Vienna Drosophila RNAi Center	v9793
D. melanogaster: UAS-ImpL2, UAS-ImpL2	Kyoto Stock Center	DGRC:117649
D. melanogaster: ptc-GFP (enhancer trap)	Carnegie collection, through A. Spradling and M. Buszczak (Carnegie Institution of Washington)	CB02030
D. melanogaster: UAS-esgNLAP	(Voog et al., 2014)	N/A
D. melanogaster: esg-GFP	Lynn Cooley, Yale	P01986
D. melanogaster: esgEP2009	Berkeley Drosophila Genome Project, ((Rørth et al., 1998); (Abdelilah-Seyfried et al., 2000))	N/A
D. melanogaster: w1118	D. Walker, UCLA	FBal0018186
D. melanogaster: c587-Gal4	T. Xie (Stowers Institute of Biomedical Research)	N/A
D. melanogaster: UAS-Dam-esg	(Loza-Coll et al., 2014)	N/A
D. melanogaster: UAS-Dam	(Loza-Coll et al., 2014)	N/A
Oligonucleotides
Primer Act5C For: TTGTCTGGGCAAGAGGATCAG	(Senos Demarco et al., 2020)	N/A
Primer Act5C Rev: ACCACTCGCACTTGCACTTTC	(Senos Demarco et al., 2020)	N/A
InR Fwd: GCACCATTATAACCGGAACC	This paper	N/A
InR Rev: TTAATTCATCCATGACGTGAGC	This paper	N/A
ImpL2 Fwd: GCCGATACCTTCGTGTATCC	This paper	N/A
ImpL2 Rev: TTTCCGTCGTCAATCCAATAG	This paper	N/A
Esg Fwd: CGCCAGACAATCAATCGTAAGC	(Loza-Coll et al., 2014)	N/A
Esg Rev: TGTGTACGCGAAAAAGTAGTGG	(Loza-Coll et al., 2014)	N/A
Software and algorithms
Prism v6.0	Graphpad	N/A
Illustrator CC 2015	Adobe	N/A
Photoshop CC 2015	Adobe	N/A
Image J v2.0.0	Wayne Rasband, NIH	http://imagej.nih.gov/ij
CFX ManagerTM	Biorad	N/A