Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 19;39(3):110723. doi: 10.1016/j.celrep.2022.110723

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Overexpression of LMRG_02920 inhibits the bacterial SOS response

(A) Quantitative real-time PCR analysis of representative SOS genes (recA, lexA, and uvrX/LMRG_02221) in WT Lm and Lm bacteria expressing LMRG_02920 (using pPL2-2920). Indicated strains were grown in BHI medium with MC treatment at 30°C for 45 min. mRNA levels are presented as RQ relative to their levels in WT bacteria. The error bars represent the standard deviation of three independent experiments.

(B) Quantitative real-time PCR analysis of representative SOS genes (recA, lexA, and uvrX/LMRG_02221) in Δφ/Δmon and Δφ/Δmon bacteria expressing LMRG_02920 (using pPL2-2920). Indicated strains were grown in BHI with MC treatment at 30°C for 45 min. mRNA levels are presented as RQ relative to their levels in WT bacteria. The error bars represent the standard deviation of three independent experiments.

(C) Quantitative real-time PCR analysis of representative SOS genes (lexA and uvrX/LMRG_02221) in WT Lm and lexA3 bacteria expressing or not expressing recA from pPL2 plasmid (pPL2-recA). Indicated strains were grown in BHI medium with MC treatment at 30°C for 45 min. mRNA levels are presented as RQ relative to their levels in WT bacteria. The error bars represent the standard deviation of three independent experiments.

(D) A plaque-forming assay of φ10403S virions produced by WT Lm and lexA3 bacteria expressing or not expressing recA from pPL2 plasmid (pPL2-recA). Virions obtained from MC-treated bacterial cultures (6 h after MC treatment at 30°C) were tested on an indicator strain for plaque formation (numerated as PFUs). The data are presented as percentage of the levels measured for WT Lm. The experiment was performed three times and error bars represent the standard deviation of independent experiments.

(E) A plaque-forming assay of φ10403S virions produced by WT Lm and bacteria expressing recA and LMRG_02920 (alone or together) from the pPL2 plasmid. Virions obtained from MC-treated bacterial cultures (6 h after MC treatment at 30°C) were tested on an indicator strain for plaque formation (numerated as PFUs). The experiment was performed three times, and the error bars represent the standard deviation of three independent experiments.

(F) Quantitative real-time PCR analysis of representative SOS genes (lexA and uvrX/LMRG_02221) in WT Lm and in bacteria expressing recA and LMRG_02920 (alone or together) from pPL2 plasmid. Indicated strains were grown in BHI medium with MC treatment, at 30°C, for 45 min. mRNA levels are presented as RQ, relative to their levels in WT bacteria. The error bars represent the standard deviation of three independent experiments.

(G) Virions obtained from lytic induction of WT Lm were used to infect Δφ/ΔcomK, Δφ/ΔcomK expressing LMRG_02920 (Δφ/ΔcomK + pPL2-2920), and Δφ/ΔcomK expressing the φ10403S main repressor (Δφ/ΔcomK + pPL2-1514) as a control. The number of virions produced upon infection is presented as PFU/mL. The experiment was performed three times, and the error bars represent the standard deviation of independent experiments.