Figure 7.

AriS affects only the phage and not the mon under MC treatment

(A) A plaque-forming assay of φ10403S. Virions were obtained from MC-treated cultures (6 h after the addition of MC) of WT Lm, ΔariS, and bacteria deleted of each AriS domain separately: ΔantA/B and ΔkilAC. Virions were used to infect an indicator strain and are numerated as PFUs. Error bars represent the standard deviation of three independent experiments.

(B) A monocin killing assay performed with monocins obtained from MC-treated bacterial cultures (6 h after MC treatment) of WT Lm, ΔariS, ΔantA/B, and ΔkilAC. A diluted overnight culture of the indicator Lm strain ScottA was supplemented with serial dilutions of filtered supernatants containing monocins and was grown at 30°C. OD₆₀₀ was measured after 10 h. The error bars represent the standard deviation of three independent experiments.

(C) Quantitative real-time PCR analysis of uvrX in WT Lm and indicated mutants. The strains were grown in BHI medium with MC treatment at 30°C for 45 min. mRNA levels are presented as RQ relative to their levels in WT bacteria. The error bars represent the standard deviation of three independent experiments.