Anti-bleaching buffer 3× stock [125 μL]

Reagent	Final concentration	Amount
H2O	/	65 μL
Methyl cellulose	0.375%	23.5 μL
BRB80 20×	BRB80 1×	6.25 μL
D-glucose (15 mg/mL)	0.9 mg/mL	7.5 μL
Glucose oxidase (5 mg/mL)	0.3 mg/mL	7.5 μL
Catalase (1 mg/mL)	0.06 mg/mL	7.5 μL
DTT (1 M)	30 mM	3.75 μL
GTP (0.1 M)	3 mM	4 μL

Store on ice for 4 h.