FIG. 5.

Alignment of the amino acid sequence of KPC-1 with that of Sme-1 from S. marcescens S6 (41), Nmc-A from E. cloacae NOR1 (42), and IMI-1 from E. cloacae (57). Dashes indicate the gaps that were inserted to optimize the alignment. The numbering is from Ambler et al. and Sykes (1, 67). The conserved domains of class A β-lactamases are underlined (12, 26, 35, 36, 55, 63, 66). The residues suggested to play a critical role for carbapenemase activity are marked by asterisks and underlined (63, 66). The positions where the KPC-1 sequence diverges from these conserved residues (positions 105 and 237) are indicated in italics.