TABLE 3.

Substrate profile for KPC-1

Substrate	kcat (s−1)	Relative kcat	Km (μM)	Relative kcat/Km
Cephaloridine	340 ± 16	100	560 ± 97	100
Cephalothin	75 ± 7	22	53 ± 0.3	240
Nitrocefin	78 ± 2	23	24 ± 5	530
Cefotaxime	14 ± 1	4.2	160 ± 19	15
Cefoxitin	0.26 ± 0.01	0.08	120 ± 17	0.4
Ceftazidime	0.10 ± 0.02	0.03	94 ± 1.8	0.2
Benzylpenicillin	32 ± 1	9.6	23 ± 2.3	230
Ampicillin	110 ± 4	33	130 ± 21	140
Cloxacillin	25 ± 1	7.4	100 ± 35	41
Piperacillin	24 ± 0	7.2	18 ± 3	230
Imipenem	12.4 ± 0	3.7	81 ± 0.4	26
Meropenem	3.0 ± 0.1	0.9	12 ± 2	41.5
Aztreonam	20 ± 1	5.9	310 ± 35	11