Table 1.
Antibodies for flow cytometric analysis.
| Panel | Surface staining | Conjugate | Intracellular staining | References |
|---|---|---|---|---|
| I | CD4 biotinylateda clone HB16A1 | PE Streptavidin3 | (32) | |
| CD8 DyL405bclone CVS82 | CD154 DyL488c clone 5C84 | (25, 27) | ||
| IL-4 AF647d clone 13G72 | (33) | |||
| II | CD4 biotinylateda clone HB16A1 | PE Streptavidin3 | (32) | |
| CD154 VioBlue clone 5C85 | (25, 27) | |||
| TNF-a DyL488c clone 48-42, | (34) | |||
| IFN-g AF647d clone 38-12 | (5) |
Antibody and conjugate sources: 1 Washington State University, Pullman, WA, USA; 2 Dr. B. Wagner, Cornell University, Ithaca, NY, USA; 3 BioLegend®, San Diego, CA, USA; 4 Hoelzel, Cologne, Germany; 5 Miltenyi Biotec, Bergisch Gladbach, Germany; conjugated with. a EZ-Link™ Sulfo-NHS Biotin. b DyLight™ 405 NHS Ester. c DyLight™ 488 NHS Ester, or d Alexa Fluor™ 647 NHS Ester, (all Thermo Fisher Scientific, Rockford, IL, USA).