Table 2.
Tetanus toxoid-specific serum immunoglobulin ELISA specifications.
| Ig/isotype | Pan-Ig | IgG1 | IgG3/5 | IgG4/7 |
|---|---|---|---|---|
| Detection antibody | Biotin-SP AffiniPure Goat Anti-Horse IgG (H+L) | Anti-IgG1 CVS45 biotinylated* | Anti-IgG3/5 586 biotinylated* | Anti-IgG4/7 CVS39 biotinylated* |
| Reference | JacksonImmuno Research 108-065-003 | (37, 38) | (38) | (37, 38) |
| Purified Ig standard, Maximum Ig concentration | pH 2 eluate, 5 µg/ml | pH 3 eluate, 10 µg/ml | pH 2 eluate, 10 µg/ml | pH 2 eluate, 5 µg/ml |
| Test sera dilution | 1:4000 | 1:500 | 1:4000 | 1:1000 |