Figure 6.

Dissecting killing mechanisms of T_EM and T_CM. (A) To analyze target cell lysis by perforin-mediated or FasR-mediated killing mechanisms, sorted T_EM or T_CM and target cell were either un-treated as control (A) or treated with inhibiting FasL antibodies NOK-1 and NOK-2 (10 µg/each) (B), with 50 nM CMA (C) and with inhibiting FasL antibodies and CMA in combination (D). Effects of treatments on T_EM and T_CM are shown as representative death plots, respectively (A-D). (E, F) Quantification of target cell lysis at 2h, 4h and 8h for T_EM (E) or T_CM (F). (G) Δt of apoptosis for each analyzed target cell lysed by treated T_EM or T_CM in comparison to untreated (T_EM or T_CM) control cells. (H) Impact of FasL blocking quantified by analysis of area under the curve (AUC). Each point reflects the Δt of apoptosis induction from a single target cell. n=4 donors, T_EM 94 cells, +αFasL 89 cells, +CMA 103, +CMA+αFasL (n=2 donors) 79, T_CM 105 cells, +αFasL 122 cells, +CMA (n=2 donors) 122 cells, +CMA+αFasL 102 cells. Statistical analysis was done using a two-way ANOVA. *p<0.05; **p<0.01; ***p<0.001; ns, no significant difference.