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Proteolytic Inhibitor Solution
| Reagent | Stock concentration | Final concentration | Amount |
|---|---|---|---|
| Bovine Serum Albumin | N/A | 0.15 mM | 150 mg |
| Ovomucoid | N/A | 0.357 mM | 150 mg |
| Balanced Salt Solution | N/A | N/A | 15 mL |
| Total | N/A | N/A | 15 mL |
Sterilize using a 0.22 μm filter and store at room temperature (20°C) prior to use. Prepare the inhibitor solution fresh for each experiment and discard the unused solution. Prepare enzyme inhibitor solution fresh for each experiment and discard the unused enzyme solution.
