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. 2022 Apr 13;13:846524. doi: 10.3389/fmicb.2022.846524

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Copyright © 2022 Hui, Guo, Li, Chen and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of Cd(II) at a constant concentration on the response of dual-sensing biosensor toward Hg(II) at varied concentrations from 0 to 10 μM. Dual-sensing TOP10/pR-Pcad-Pmer-G in lag phase were exposed to 100 μM Cd(II) in the presence of increased concentrations of Hg(II). After incubation at 37^oC for 8 h, bacterial cell densities (A) were determined immediately. The inset is a representative photo of induced cultures. Double-color fluorescence derived from TOP10/pR-Pcad-Pmer-G was then determined (B), and the mean of fluorescence intensity ratios (eGFP/mCherry) were shown in the table below the corresponding figure. Data represent the mean ± SD of at least three independent experiments.