TABLE 1.
Strain and vectors | Genotypes or description | References |
Strain | ||
E. coli TOP10 | F–, Φ80lacZΔM15, ΔlacX74, recA1 | Invitrogen |
Plasmids | ||
pET-21a | AmpR, T7 promoter, lac operator | Novagen |
pT-RFP | T vector carrying mcherry | Hui et al., 2018c |
pT-GFP | T vector carrying egfp | Hui et al., 2018c |
pPmer | pET-21a derivative containing merR and Pmer divergent promoter region cloned into BglII and XbaI sites | Hui et al., 2021c |
pPcad | pET-21a derivative containing cadR and Pcad divergent promoter region cloned into BglII and XbaI sites | Hui et al., 2021c |
pPmer-G | pPmer derivative carrying promoterless egfp and rrnB terminator cloned into NdeI and HindIII sites | This study |
pR-Pmer-G | pPmer-G derivative, a promoterless mcherry cassette fused to the downstream of merR | This study |
pPcad-R | pPcad derivative carrying promoterless mcherry and rrnB terminator cloned into NdeI and XhoI sites | This study |
pG-Pcad-R | pPcad-R derivative, a promoterless egfp cassette fused to the downstream of cadR | This study |
pR-Pcad | pET-21a derivative containing cadR-Pcad-mcherry cloned into BamHI and BglII sites | This study |
pR-Pcad-Pmer-G | pR-Pcad derivative containing merR-Pmer-egfp cloned into BamHI and HindIII sites | This study |