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. 2022 Apr 13;13:846524. doi: 10.3389/fmicb.2022.846524

TABLE 1.

Bacterial strain and plasmids used in this study.

Strain and vectors Genotypes or description References
Strain
E. coli TOP10 F, Φ80lacZΔM15, ΔlacX74, recA1 Invitrogen
Plasmids
pET-21a AmpR, T7 promoter, lac operator Novagen
pT-RFP T vector carrying mcherry Hui et al., 2018c
pT-GFP T vector carrying egfp Hui et al., 2018c
pPmer pET-21a derivative containing merR and Pmer divergent promoter region cloned into BglII and XbaI sites Hui et al., 2021c
pPcad pET-21a derivative containing cadR and Pcad divergent promoter region cloned into BglII and XbaI sites Hui et al., 2021c
pPmer-G pPmer derivative carrying promoterless egfp and rrnB terminator cloned into NdeI and HindIII sites This study
pR-Pmer-G pPmer-G derivative, a promoterless mcherry cassette fused to the downstream of merR This study
pPcad-R pPcad derivative carrying promoterless mcherry and rrnB terminator cloned into NdeI and XhoI sites This study
pG-Pcad-R pPcad-R derivative, a promoterless egfp cassette fused to the downstream of cadR This study
pR-Pcad pET-21a derivative containing cadR-Pcad-mcherry cloned into BamHI and BglII sites This study
pR-Pcad-Pmer-G pR-Pcad derivative containing merR-Pmer-egfp cloned into BamHI and HindIII sites This study