Fig. 1.
The activation of sirtuin 1 (SIRT1) by resveratrol (RES) improved doxorubicin (DOX)-induced cardiac dysfunction and myocardial remodeling. (A) Western blot analysis of SIRT1 expression in different organs of Sirt1flox/flox and Sirt1-CKO mice. (B–F) Cardiac function was examined by echocardiography (n = 5–6). (G) FITC-conjugated WGA staining of cardiac tissues and quantification of myocytes cross-sectional areas (n = 6). (H, I) Representative (H) Masson's trichrome and (I) Sirius red staining images of cardiac tissues and quantification of cardiac fibrotic areas (n = 6). (J, K) RT-qPCR for measuring mRNA expression of hypertrophic markers atrial natriuretic peptide (Anp) and brain natriuretic peptide (Bnp) in cardiac tissues (n = 5–6). (L) Cardiac connective tissue growth factor (Ctgf) mRNA expression was measured by RT-qPCR (n = 5–6). Data were presented as mean ± SD. *P < 0.05, ns indicates no significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)