Fig. 3.

The activation of SIRT1 attenuated DOX-induced cardiac oxidative stress. (A, D) Representative microphotographs and quantification of fluorescence intensity of DHE staining (red) in cardiac sections (n = 6). (B, C, E, F) IHC staining using (B, E) anti-4-hydroxynonenal (4-HNE) and (C, F) anti-3-nitrotyrosine (3-NT) antibody in cardiac tissues (brown considered positive staining) and related quantitative analysis (n = 6). (G–I) The protein expression of nuclear nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase-1 (HO-1) was analyzed by western blot with densitometric quantification of each group (n = 6). Histone H3 or GAPDH as an internal control. (J, K) Cardiac catalase (Cat) and superoxide dismutase (Sod) mRNA levels were tested by RT-qPCR (n = 6). (L) Nuclear accumulation of NRF2 (indicated by white arrows) determined by immunofluorescent staining with anti-NRF2 antibody (red) in cardiac tissues. Data were presented as mean ± SD. *P < 0.05, ns indicates no significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)