Fig. 4.

The activation of SIRT1 ameliorated DOX-induced oxidative stress and apoptosis in mice and H9c2 cells. (A, B) Detection of apoptotic cells by TUNEL staining with quantification of apoptosis rate in cardiac tissues (n = 6 in B). (C, D) Representative images of (C) DCFH-DA (green) and (D) DHE (red) staining and quantification of the corresponding fluorescence intensity in H9c2 cells. Three independent experiments were performed. (E) H9c2 cells were stained with DCFH-DA and analyzed by flow cytometry. Three independent experiments were performed. (F) Apoptosis rate of H9c2 cells was determined by flow cytometry analysis using annexin V/PI double staining. Three independent experiments were performed. Data were presented as mean ± SD. *P < 0.05, ns indicates no significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)