Figure 4.

Effects of GLP-1, E2, and GLP1-E2 on protein expression in human islets

Cultured human islets (N = 3 donors) were treated with GLP-1, E2, or GLP1-E2 for 5 min, 30 min, and 18 h, compared to control untreated islets, and studied by RPPA.

(A) Bioinformatic analysis of major proteins pathways by GO analysis using Enrich.

(B) Heatmap of individual proteins. Normalized values from the three individual donors were averaged to generate an average Z score and map proteins in the heatmap; ∗p < 0.05 (t test) and #p < 0.05 (Fisher’s LSD test) compared to control.