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. 2022 Apr 22;154(5):e202213131. doi: 10.1085/jgp.202213131

Figure S2.

Figure S2.

Simulated fits of WT binding isotherms. (a) WT GltPh, 500 mM Na-gluconate, 15°C; kD1 and kD2 of Fit 1 (solid) and Fit 2 (dashed) are constrained to 0.3 and 0.4 nM, respectively. Fit 1 and Fit 2 have parameters of n1, 0.82 and 0.37; ΔH1, −22.7 and −50.0 kcal mol−1; n2, 0.36 and 0.89; ΔH2, 39.4 and 16.2 kcal mol−1. (b) WT GltPh, 500 mM Na-gluconate, 35°C; kD1 and kD2 are constrained to 1.6 and 3.6 nM, respectively. Fit has parameters of n1, 0.16; ΔH1, −24.6 kcal mol−1; n2, 1.30; ΔH2, −4.7 kcal mol−1.