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. 2022 Feb 22;71(5):1149–1165. doi: 10.2337/db21-0830

Figure 5.

Figure 5

TNT-mediated overexpression of PLCγ2 improves perfusion in nondiabetic ischemic limbs. A: Schematic diagram showing TNT-assisted delivery of PLCγ2 ORF in murine ischemic limbs. Schematic diagram was created with BioRender.com. B: Western blot analysis of TNT-mediated PLCγ2 overexpression in murine skin. Data represent 10 different sites from 3 mice. *P < 0.05 (Student t test). Data are represented as the mean ± SEM. PeriMed laser speckle–assisted perfusion images (C) and their analysis (D) in ischemic limbs on which TNT procedure was done with sham or PLCγ2 ORF in nondiabetic conditions. Perfusion was calculated based on the ratio of the ischemic versus normal/contralateral limb. n = 6 to 7. *P < 0.05 (Student t test); **P < 0.005. E: Immunohistochemical analysis of CD31+/VWF+. G: CD31+/SMA+ colocalization in ischemic limbs of mice treated with sham or PLCγ2 ORF at day 14 postsurgery and their colocalization coefficient (F and H) (n = 5–7). *P < 0.05, Student t test. Data are represented as the mean ± SEM. AU, arbitrary unit.