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. 2022 Feb 22;71(5):1149–1165. doi: 10.2337/db21-0830

Figure 7.

Figure 7

Endothelial cell–targeted delivery of PLCG2 augments VEGF therapy rescue of the T2DM (db/db) diabetic ischemic limb. A: Schematic diagram showing TNT-mediated delivery of VEGF ORFs or/and endothelial PLCγ2 ORFs in the ischemic hindlimb of db/db diabetic mice. B: Blood glucose level in db/db mice used in this study. TNTsham (n = 6; 4 male and 2 female), TNTVEGF (n = 7; 4 male and 3 female), and TNTVEGF+PLCG2 (n = 6; 3 male and 3 female). PeriMed laser speckle–assisted perfusion images (C) and their analysis (D) in ischemic limbs on which TNT procedure was done with sham, VEGF only, or VEGF plus CDH5 promoter–driven (endothelial) PLCγ2 cocktail. Perfusion was calculated based on the ratio of the ischemic vs. normal/contralateral limb. n = 6 to 7. *P < 0.05 TNTVEGF vs. TNTsham; §P < 0.05 TNTVEGF+PLCγ2 vs. TNTsham; #P < 0.05 TNTVEGF+PLCγ2 vs. TNTVEGF (one-way ANOVA, followed by Tukey HSD post hoc test). Immunohistochemical analysis of CD31+/VWF+ colocalization in diabetic ischemic limbs of db/db mice treated with above-mentioned cocktails at day 14 postsurgery (E) and the colocalization coefficient (F). n = 6. *P < 0.05 (one-way ANOVA, followed by Tukey HSD post hoc test). AU, arbitrary unit.