TABLE 5.
Interaction parameters (I10, I50, I90) for 10, 50, and 90% inhibition of DNA synthesis of wt and L100I mutant HIV-1 RT by combinations of different NNRTIsa
| RT | Drug (d1-d2)b | Mean ± SD
|
|||||
|---|---|---|---|---|---|---|---|
| Econ (%) | D1 (μM) | D2 (μM) |
I
|
||||
| 10 | 50 | 90 | |||||
| wt | NVP-EFV | 103 ± 5 | 0.16 ± 0.02 | 0.02 ± 0.005 | 1.2 ± 0.1 | 0.72 ± 0.02 | 0.26 ± 0.02 |
| L100I | NVP-EFV | 93 ± 5 | 4.2 ± 0.1 | 0.023 ± 0.002 | 0.9 ± 0.1 | 0.72 ± 0.02 | 0.15 ± 0.01 |
a
I10, I50, and I90, interaction index at 10, 50, and 90% inhibition of RT activity, respectively; Econ, estimated parameter for the control effect in the absence of the drug; D1 and D2, dose of inhibitors giving 50% inhibition of RT activity when tested in the combination (D1 + D2). For details see Materials and Methods.
b
NVP, nevirapine; EFV, efavirenz. d1 and d2, drugs 1 and 2 in the combination.