FIGURE 8.

Reduced expression of Unc‐45 in Drosophila increases intestinal permeability. (A) Immunoblotting analysis of Unc‐45 protein in wild‐type (w1118) or heterozygous unc‐45 mutant (unc‐45^EY03043‐33 or unc‐45⁰³⁶⁹²) Drosophila melanogaster adult flies. β‐tubulin was used as a loading control. (B) Pictures of adult Drosophila females categorized as non‐Smurf (blue dye localized to intestine and proboscis) or Smurf (blue dye spread from intestine to the majority of tissues). (C) Quantitation of the percentage of flies exhibiting the Smurf phenotype (right picture in B) for each gender of each genotype analyzed in A. Each dot represents an individual experiment containing 25 flies. *p < .05, **p < .01