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. Author manuscript; available in PMC: 2022 Apr 27.
Published in final edited form as: J Mol Biol. 2021 Oct 8;433(23):167295. doi: 10.1016/j.jmb.2021.167295

Table 1.

Parameters derived from ATPase and single ATP turnover studies

IC50/ EC50 (in μM) derived using data acquired in various bovine cardiac systems from ATPase and Single ATP turnover experiments
Para-nitroblebbistatin Mavacamten
0μM
blebbistatin		 10μM
blebbistatin
Basal ATPase (BcSTF) 2.37±0.12* 0.63±0.05
Actin-activated ATPase (BcSTF) 4.81±0.16* 1.25±0.11
Myofibrillar ATPase (BcMF) 4.05±0.34* 0.50±0.05
SRX Amplitude, Aslow (BcSTF) >12.5* 1.21±0.35 0.63±0.07*
DRX ATPase Rate, kfast (BcSTF) >12.5* 0.43±0.03 0.64±0.12
SRX ATPase Rate, kslow (BcSTF) >12.5 >12.5 >12.5
SRX Amplitude, Aslow (BcMF) >12.5* 4.30±0.31
DRX ATPase Rate, kfast (BcMF) >12.5* 0.59±0.08
SRX ATPase Rate, kslow (BcMF) >12.5 >12.5

Nomenclature is as follows: BcSTF refers to bovine β-cardiac synthetic thick filaments; BcMF, bovine cardiac myofibrils. Data are expressed as mean±SEM (n≥8 from two experiments that used single preparation of the same purification). The IC50/EC50 values of both blebbistatin and para-nitroblebbistain are statistically different from mavacamten based on a student’s t-test (*p<0.001). The Hill coefficient measured in these experiments is always between 2–4.