Fig. 3.

Knockdown of TLNC1 represses tumor growth and metastasis of hepatoma cells. a qPCR quantification of the expression levels of TLNC1 in SNU449 cells transfected with control shRNA or shRNAs targeting TLNC1. b Cell viability of indicated SNU449 cells was measured by CCK-8 assay. c Cell proliferation of indicated SNU449 cells was examined by EdU assay. d Cell migration of indicated SNU449 cells was measured by wound healing assay. e-f Cell migration and invasion of indicated SNU449 cells were measured by transwell migration and matrigel invasion assays, respectively. The data are the means ± SEM and are representative of three independent experiments. g Effects of TLNC1 knockdown in HCCLM3 and SK-Hep1 cells on subcutaneous tumor growth. Tumor volumes and tumor weights were measured after the mice were killed (n = 5). h The number of tumor nodules formed in the livers of orthotopic-implantation models inoculated with indicated hepatoma cells was indicated in the bar graph (n = 5). i The number of metastatic foci formed in the lungs of lung metastasis models inoculated with indicated hepatoma cells was indicated in the bar graph (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001