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. 2022 Apr 27;21:105. doi: 10.1186/s12943-022-01578-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TLNC1 interacts with TPR and represses the transcriptional activity of p53. a Validation of nucleotide sequences in TLNC1 that bind to TPR. Upper panel, nucleotide electrophoresis showing the sizes of full-length and truncated TLNC1. Lower panel, immunoblot analysis of TPR in protein samples pulled down by full-length or truncated TLNC1. b Immunoblotting of the full-length or truncated Flag-TPR in the lysates from 293 T cells transfected with the indicated vectors, or in the retrieved proteins by the biotinylated TLNC1 probe from the lysates of 293 T cells transfected with the indicated vectors. c Validation of amino acid sequences in TPR that bind to TLNC1. Left panel, diagrams of full-length and truncated Flag-TPR. Right panel, qRT-PCR analysis after RIP assays of SK-Hep1 cells transfected with full-length and truncated Flag-TPR. d Dual luciferase assays for SK-Hep1 cells transfected with control or TLNC1 vectors and HCCLM3 cells transfected with control shRNA or shRNA targeting TLNC1. e qRT-PCR analysis for the expression of downstream targets of p53 signaling in indicated SK-Hep1 (TLNC1 overexpression) and HCCLM3 (TLNC1 knockdown) cells. f Above, immunoblot analysis of TPR and p53 in indicated SK-Hep1 cells treated with or without siTPR. Below, qRT-PCR detection of TLNC1 in indicated SK-Hep1 cells treated with or without siTPR. g qRT-PCR analysis for the expression of p21 in indicated SK-Hep1 cells. h Cell viability of indicated SK-Hep1 cells was measured by CCK-8 assay at 5 days post seeding. i Cell migration and invasion of indicated SK-Hep1 cells were measured by transwell migration and matrigel invasion assays, respectively. The data are the means ± SEM and are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001