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. 2022 Apr 27;21:105. doi: 10.1186/s12943-022-01578-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — TLNC1 represses nuclear translocation of p53 through interaction with TPR. a p53 expression in indicated SK-Hep1 and HCCLM3 cells, as detected by an immunofluorescence assay. The merged images show overlays of p53 (green) and nuclear staining by DAPI (blue); scale bar: 20 μm. b Immunoblot analysis of p53 in indicated SK-Hep1 and HCCLM3 cells. c p53 expression in cytoplasmic and nuclear fractions, as detected by immunoblot analysis. GAPDH was used as a loading control for the cytoplasmic fraction, and PARP was used as a loading control for the nuclear fraction. d Co-immunoprecipitation among TPR, CRM1 and p53 in SK-Hep1 and HCCLM3 cells, as detected by immunoblot analysis. e Co-immunoprecipitation among TPR, CRM1 and p53 in indicated SK-Hep1 (TLNC1 overexpression) and HCCLM3 (TLNC1 knockdown) cells, as detected by immunoblot analysis. f Co-immunoprecipitation among recombinant TPR, CRM1 and p53 proteins, as detected by immunoblot analysis. The data are the representative of three independent experiments. g Surface plasmon resonance analysis of the binding of CRM1 with increasing concentrations of recombinant TPR in the presence or absence of TLNC1