Table 1.
Gene Ontology analysis of significantly altered biological processes in 2DG treated murine heart indicates that the principal effects of the compound are to augment the expression of proteins related to the respiratory chain and ATP synthesis.
| GO biological process complete | Fold enrichment | Raw P-value | FDR < 0.05 |
|---|---|---|---|
| Analysis type: B/A | |||
| Mitochondrial electron transport, NADH to ubiquinone (GO:0006120) | 76.35 | 4.54E-07 | 3.57E-03 |
| Purine ribonucleoside biosynthetic process (GO:0046129) | 51.53 | 3.96E-05 | 4.45E-02 |
| Nucleoside biosynthetic process (GO:0009163) | 51.53 | 3.96E-05 | 4.16E-02 |
| Ribonucleoside biosynthetic process (GO:0042455) | 51.53 | 3.96E-05 | 3.90E-02 |
| Purine nucleoside biosynthetic process (GO:0042451) | 51.53 | 3.96E-05 | 3.67E-02 |
| Alternative mRNA splicing, via spliceosome (GO:0000380) | 46.85 | 5.12E-05 | 4.48E-02 |
| Glycosyl compound biosynthetic process (GO:1901659) | 44.81 | 5.78E-05 | 4.79E-02 |
| Mitochondrial ATP synthesis coupled electron transport (GO:0042775) | 33.68 | 5.98E-07 | 3.14E-03 |
| ATP synthesis coupled electron transport (GO:0042773) | 31.23 | 8.48E-07 | 3.34E-03 |
| Oxidative phosphorylation (GO:0006119) | 23.53 | 3.15E-06 | 7.08E-03 |
| Respiratory electron transport chain (GO:0022904) | 23.53 | 3.15E-06 | 6.20E-03 |
| Electron transport chain (GO:0022900) | 22.31 | 4.03E-06 | 7.06E-03 |
| ATP metabolic process (GO:0046034) | 18.08 | 2.46E-09 | 3.88E-05 |
| Cellular respiration (GO:0045333) | 13.63 | 3.94E-05 | 4.78E-02 |
| Energy derivation by oxidation of organic compounds (GO:0015980) | 11.02 | 2.04E-05 | 2.68E-02 |
| Analysis type: C/A | |||
|---|---|---|---|
| Intracellular distribution of mitochondria (GO:0048312) | 67.86 | 2.10E-05 | 2.07E-02 |
| Mitochondrial electron transport, NADH to ubiquinone (GO:0006120) | 45.24 | 6.04E-05 | 4.75E-02 |
| NADH dehydrogenase complex assembly (GO:0010257) | 22.16 | 4.30E-05 | 3.98E-02 |
| Mitochondrial respiratory chain complex I assembly (GO:0032981) | 22.16 | 4.30E-05 | 3.76E-02 |
| Regulation of protein import (GO:1904589) | 16.76 | 1.62E-05 | 1.70E-02 |
| Cellular respiration (GO:0045333) | 12.93 | 9.18E-06 | 1.31E-02 |
| Energy derivation by oxidation of organic compounds (GO:0015980) | 10.16 | 7.31E-06 | 1.28E-02 |
Upper section 2DG treated group B vs. controls (A), based on 64 significantly altered proteins. Lower section, 2DG treated group C vs. A (81 proteins). The peptide abundances were converted to log2 and a t-test applied and filtered, criteria for inclusion: FDR < 0.05; > tenfold enrichment. GO processes in bold relate to mitochondrial energy production and ATP metabolism.