Table 3.

Surface charge and potential from surface force measurements. Fraction of charged lipids dissociated was calculated as described in Table 2.

Outer leaflet composition (mol%)	σ (mC/m2)	ψ (mV)	Area per charge (nm2 per e−)	Fraction of charged lipids dissociated (Approx.%)	Overall lipids dissociated (%)
10:90 DMPG:DMPC	−2.9 ± 0.6	−50 ± 5	55 ± 10	8	0.8
20:80 DMPG:DMPC	−4.0 ± 0.5	−64 ± 5	40 ± 5	6	1.2
100:0 DMPG:DMPC	−5.3 ± 0.6	−75 ± 6	30 ± 3	1.5	1.5 ± 0.2
10:90 DMPS:DMPC	−3.4 ± 0.6	−57 ± 5	47 ± 7	10	1
20:80 DMPS:DMPC	−3.5 ± 0.5	−58 ± 5	46 ± 6	5	1
100:0 DMPS:DMPC	−4.0 ± 0.3	−64 ± 3	40 ± 3	1.1	1.1 ± 0.1