Fig. 3. (a)Photomicrographs of pancerase stained by hematoxylin and eosin, toluidine blue and insulin immunohistochemical staining. (b) The diameter of pancreatic islets (μm). (c) Immunohistochemical analysis for area% of insulin expression. Data expressed as mean ± SD (**P < 0.01, ***P < 0.001). Notable arrows on the figure indicate necrobiotic changes in β-cells.
